After decomposing the sample by dry method, dissolve the residue with sulfuric acid and ammonium sulfate, and test it. 试样以干法分解,以硫酸,硫酸铵溶解残渣后进行测试。
The goat pox virus ( GTPV) has been purified through ammonium sulfate sedimentation and Sephadex G-200 chromatography. The sheep erythrocyte was sensitized with purified virus and the indirect hemagglutination test ( IHA) was developed. 采用硫酸铵盐析、SephadexG-200柱层析的方法纯化山羊痘病毒,以病毒抗原致敏绵羊红细胞建立了山羊痘正向间接血凝试验,并进行了最佳试验条件的摸索。
Polyclonal antibodies against RCT have been raised in three rabbits and purified by using a saturated ammonium sulfate method and by the way of protein A-Sepharose 4B. The sensitivity and cross-reactivity of polyclonal antibody have been demonstrated in ELISA test. 通过免疫兔子获得了RCT的多克隆抗体,然后又通过饱和硫铵沉淀和ProteinA-Sepharose4B的方法对抗体进行了纯化。
Then the feather meal concentration, temperature, initial pH, inoculum size, the additional Ammonium Sulfate and maltose were optimized by orthogonal test based on the single-factor test. 在单因子试验的基础上采用正交试验设计对底物浓度、温度、初始pH、接种量、外加(NH4)2SO4、外加麦芽糖进行优化。